The simplest strategy to obtain the d-spacing values is by directly measuring the distance between atomic layers of the nanoparticle from the high-resolution image: Therefore, in this post I would like to summarize different strategies using ImageJ to obtain d-spacing values from HR-TEM images, which may be useful for someone else, particularly for beginners. There is a specific plugin for automatically determine the d-spacing values from TEM images, but it has not been working for me. ImageJ is an excellent alternative for analyzing all kind of images, and it is open source and modular with a lot of powerful plugins, macros and so on. Some tutorials have addressed how to run this software using wine, but just for quite old versions. It is great that they allow to use an offline version for free, but unfortunately, it does not run on GNU/Linux or Mac OS X. I have been analyzing some High-Resolution Transmission Electron Microscopy (HR-TEM) images of metallic nanoparticles recently, and one of the most useful properties to obtain is the d-spacing values from the crystalline structure of individual nanoparticles (or for the whole sample).Īs you may know, Gatan’s Digital Micrograph is the most popular software package for analyzing electron micrographs, and it makes to obtain d-spacing values quite simple.
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